Funded by a grant from the National Center for Research Resources (NCRR) (5P40RR003640),
National Institutes of Health
Sabana Seca
Laboratory of Reproductive Biology
Program Coordinator
Assistant Investigator
Janis González, PhD
The main objectives of the Laboratory Reproductive Biology are to:
- produce pre-implantation embryos of genetically-characterized rhesus macaques,
- use assisted reproductive technologies (ART) to improve conception rates among non-reproductive monkeys, and
- use ART to produce rhesus macaque embryos with specific characteristics and disease predilections.
The laboratory is located in a 750 sq ft research and diagnostics laboratory. The culture room consists of an 80 sq ft room adjacent to an aseptic surgical suite.
The laboratory is equipped with an inverted microscope (Nikon TE 300) with Hoffman optics and environment control system mounted on an anti-vibration table, stereoscopic microscopes (Nikon SMZ-U and SMZ-10A), incubators (Forma 3230 Dual Chamber Incubator w/ N2 and CO2 gas guards), video recording system for sperm motion assessment (Hamatsu CCD high resolution B&W video camera Sony 13" high resolution B&W video monitor, Panasonic 1/2" Time Lapse Recorder VCR AG-6730, diagnostic ultrasound system (Shimadzu Model SDU-350A XL with 3.5-6 Mhz 15Rmm Microconvex probe and 4-8 Mhz 11Rmm Endocavity / 150° probe), IVF Micromanipulator 2 Side Set (NT-88NE) includes 2 hydraulic fine control, 2 motorized coarse control, 2 IM 6 microinjector, mounting adapter for intracytoplasmic sperm injection (ICSI).
